Selected Recent Publications

  • Skelly, D. T., Harding, A. C., Gilbert-Jaramillo, J., Knight, M. L., Longet, S., Brown, A., Adele, S., Adland, E., Brown, H., … & W James. (2021a). Two doses of SARS-CoV-2 vaccination induce more robust immune responses to emerging SARS-CoV-2 variants of concern than does natural infection. Nature communications. 2021 Aug 17;12(1):1-2 https://www.nature.com/articles/s41467-021-25167-5
  • Mendonça, L., Howe, A., Gilchrist, J. B., Sun, D., Knight, M. L., Zanetti-Domingues, L. C., Bateman, B., Krebs, A.-S., Chen, L., Radecke, J.,… W James & P Zhang. (2021). SARS-CoV-2 assembly and egress pathway revealed by correlative multi-modal multi-scale cryo-imaging. https://doi.org/10.2139/ssrn.3733984
  • Lee, H., Flynn, R., Sharma, I., Haberman, E., Carling, P. J., Nicholls, F. J., Stegmann, M., Vowles, J., Haenseler, W., Wade-Martins, R., James, W. S., & Cowley, S. A. (2020a). LRRK2 Is Recruited to Phagosomes and Co-recruits RAB8 and RAB10 in Human Pluripotent Stem Cell-Derived Macrophages. Stem Cell Reports, 14(5), 940–955. https://doi.org/10.1016/j.stemcr.2020.04.001
  • Honarmand Ebrahimi, K., Vowles, J., Browne, C., McCullagh, J., & James, W. S. (2020). ddhCTP produced by the radical-SAM activity of RSAD2 (viperin) inhibits the NAD+-dependent activity of enzymes to modulate metabolism. FEBS Letters, 594(10), 1631–1644. https://doi.org/10.1002/1873-3468.13778
  • Huo, J., le Bas, A., Ruza, R., … Javier Gilbert-Jaramillo, Michael L. Knight, …William James, Alain R. Townsend, David I. Stuart, Raymond J. Owens & Naismith, J. H. (2020a). Neutralizing nanobodies bind SARS-CoV-2 spike RBD and block interaction with ACE2. Nature Structural and Molecular Biology, 27(9), 1–9.https://doi.org/10.1038/s41594-020-0469-6
  • Cahill, T. J. T. J., Sun, X., Ravaud, C., Villa del Campo, C., Klaourakis, K., Lupu, I.-E. I.-E. I.-E., Lord, A. M. A. M. A. M., Browne, C., Jacobsen, S. E. W. S. E. W., Greaves, D. R. D. R., Jackson, D. G., Cowley, S. A., James, W., Choudhury, R. P., Vieira, J. M. J. M., Riley, P. R. P. R., del Campo, C. V., Klaourakis, K., Lupu, I.-E. I.-E. I.-E., … Riley, P. R. P. R. (2021). Tissue-resident macrophages regulate lymphatic vessel growth and patterning in the developing heart. Development, 148(3), dev194563. https://doi.org/10.1242/dev.194563
Full list

2021 publications (to August)

Angyal, A., Longet, S., Moore, S., Payne, R. P., Harding, A., Tipton, T., Rongkard, P., Ali, M., Hering, L. M., Meardon, N., & others. (2021). T-Cell and Antibody Responses to First BNT162b2 Vaccine Dose in Previously SARS-CoV-2-Infected and Infection-Naive UK Healthcare Workers: A Multicentre, Prospective, Observational Cohort Study. Lancet (London, England), in press. https://papers.ssrn.com/sol3/papers.cfm?abstract_id=3812375

Burman, R. J., Watson, L. M., Smith, D. C., Raimondo, J. V., Ballo, R., Scholefield, J., Cowley, S. A., Wood, M. J. A., Kidson, S. H., & Greenberg, L. J. (2021). Molecular and electrophysiological features of spinocerebellar ataxia type seven in induced pluripotent stem cells. PLoS ONE, 16(2 February). https://doi.org/10.1371/journal.pone.0247434

Cahill, T. J. T. J., Sun, X., Ravaud, C., Villa del Campo, C., Klaourakis, K., Lupu, I.-E. I.-E. I.-E., Lord, A. M. A. M. A. M., Browne, C., Jacobsen, S. E. W. S. E. W., Greaves, D. R. D. R., Jackson, D. G., Cowley, S. A., James, W., Choudhury, R. P., Vieira, J. M. J. M., Riley, P. R. P. R., del Campo, C. V., Klaourakis, K., Lupu, I.-E. I.-E. I.-E., … Riley, P. R. P. R. (2021). Tissue-resident macrophages regulate lymphatic vessel growth and patterning in the developing heart. Development, 148(3), dev194563. https://doi.org/10.1242/dev.194563

Clark, J. J., Sharma, P., Bentley, E., Harding, A. C., Kipar, A., Neary, M., Box, H., Hughes, G., Patterson, E. I., Sharp, J., & others. (2021). Naturally-acquired immunity in Syrian Golden Hamsters provides protection from re-exposure to emerging heterosubtypic SARS-CoV-2 variants B. 1.1. 7 and B. 1.351. BioRxiv.

Dejnirattisai, W., Zhou, D., Ginn, H. M., Duyvesteyn, H. M. E., Supasa, P., Case, J. B., Zhao, Y., Walter, T. S., Mentzer, A. J., Liu, C., Stuart, D. I., & Screaton, G. R. (2021). The antigenic anatomy of SARS-CoV-2 receptor binding domain. Cell, 184(8), 2183-2200.e22. https://doi.org/10.1016/j.cell.2021.02.032

Ebrahimi, K. H. K. H., Gilbert-Jaramillo, J., James, W. S. S. W. S., McCullagh, J. S. O. O. J. S. O. S. O., Gilbert‐Jaramillo, J., James, W. S. S. W. S., & McCullagh, J. S. O. O. J. S. O. S. O. (2021). Interferon-stimulated gene products as regulators of central carbon metabolism. FEBS Journal, 288(12), 3715–3726. https://doi.org/10.1111/febs.15625

Hall-Roberts, H., di Daniel, E., James, W. S., Davis, J. B., & Cowley, S. A. (2021a). In vitro quantitative imaging assay for phagocytosis of dead neuroblastoma cells by IPSC-macrophages. Journal of Visualized Experiments, 2021(168), 1–27. https://doi.org/10.3791/62217

Huang, K.-Y. A., Tan, T. K., Chen, T.-H., Huang, C.-G., Harvey, R., Hussain, S., Chen, C.-P., Harding, A., Gilbert-Jaramillo, J., Liu, X., Rijal, P., & Townsend, A. R. (2021). Breadth and function of antibody response to acute SARS-CoV-2 infection in humans. PLoS Pathogens, 17(2). https://doi.org/10.1371/JOURNAL.PPAT.1009352

Huo, J., le Bas, A., Ruza, R. R., Duyvesteyn, H. M. E., Mikolajek, H., Malinauskas, T., Tan, T. K., Rijal, P., Dumoux, M., Ward, P. N., & others. (2021). Author Correction: Neutralizing nanobodies bind SARS-CoV-2 spike RBD and block interaction with ACE2. Nature Structural \& Molecular Biology, 1.

Mendonça, L., Howe, A., Gilchrist, J. B., Sun, D., Knight, M. L., Zanetti-Domingues, L. C., Bateman, B., Krebs, A.-S., Chen, L., Radecke, J., & others. (2021). SARS-CoV-2 assembly and egress pathway revealed by correlative multi-modal multi-scale cryo-imaging. https://doi.org/10.2139/ssrn.3733984

Navarro-Guerrero, E., Tay, C., Whalley, J. P., Cowley, S. A., Davies, B., Knight, J. C., & Ebner, D. (2021). Genome-wide CRISPR/Cas9-knockout in human induced Pluripotent Stem Cell (iPSC)-derived macrophages. Scientific Reports, 11(1). https://doi.org/10.1038/s41598-021-82137-z

Qiang, M., Ma, P., Li, Y., Liu, H., Harding, A., Min, C., Liu, L., Yuan, M., Ji, Q., Tao, P., Shi, X., Li, Z., Wang, F., Zhang, Y., Wu, N. C., Lee, C.-C. D. C. D., Zhu, X., Gilbert-Jaramillo, J., Saxena, A., … Lerner, R. A. (2021). Potent SARS-CoV-2 neutralizing antibodies selected from a human antibody library constructed decades ago. Advanced Science, (in press). https://doi.org/10.1002/advs.202102181

Reich, M., Paris, I., Ebeling, M., Dahm, N., Schweitzer, C., Reinhardt, D., Schmucki, R., Prasad, M., Köchl, F., Leist, M., Gutbier, S., & Britschgi, M. (2021). Alzheimer’s Risk Gene TREM2 Determines Functional Properties of New Type of Human iPSC-Derived Microglia. Frontiers in Immunology, 11. https://doi.org/10.3389/fimmu.2020.617860

Skelly, D. T., Harding, A. C., Gilbert-Jaramillo, J., Knight, M. L., Longet, S., Brown, A., Adele, S., Adland, E., Brown, H., Team, M. L., & others. (2021a). Two doses of SARS-CoV-2 vaccination induce more robust immune responses to emerging SARS-CoV-2 variants of concern than does natural infection. Nature communications. 2021 Aug 17;12(1):1-2

Supasa, P., Zhou, D., Dejnirattisai, W., Liu, C., Mentzer, A. J., Ginn, H. M., Zhao, Y., Duyvesteyn, H. M. E., Nutalai, R., Tuekprakhon, A., Wang, B., Paesen, G. C., Slon-Campos, J., López-Camacho, C., Hallis, B., Coombes, N., Bewley, K. R., Charlton, S., Walter, T. S., … Screaton, G. R. (2021). Reduced neutralization of SARS-CoV-2 B.1.1.7 variant by convalescent and vaccine sera. Cell, 184(8), 2201-2211.e7. https://doi.org/10.1016/j.cell.2021.02.033

Vaughan-Jackson, A., Stodolak, S., Ebrahimi, K. H., Browne, C., Reardon, P. K., Pires, E., Gilbert-Jaramillo, J., Cowley, S. A., & James, W. S. (2021). Differentiation of human induced pluripotent stem cells to authentic macrophages using a defined, serum-free, open-source medium. Stem Cell Reports. https://doi.org/10.1016/j.stemcr.2021.05.018

Wing, P. A. C., Keeley, T. P., Zhuang, X., Lee, J. Y., Prange-Barczynska, M., Tsukuda, S., Morgan, S. B., Harding, A. C., Argles, I. L. A., Kurlekar, S., Noerenberg, M., Thompson, C. P., Huang, K. Y. A., Balfe, P., Watashi, K., Castello, A., Hinks, T. S. C., James, W., Ratcliffe, P. J., … McKeating, J. A. (2021). Hypoxic and pharmacological activation of HIF inhibits SARS-CoV-2 infection of lung epithelial cells. Cell Reports, 35(3). https://doi.org/10.1016/j.celrep.2021.109020

Zhou, D., Dejnirattisai, W., Supasa, P., Liu, C., Mentzer, A. J., Ginn, H. M., Zhao, Y., Duyvesteyn, H. M. E., Tuekprakhon, A., Nutalai, R., Wang, B., Paesen, G. C., Lopez-Camacho, C., Slon-Campos, J., Hallis, B., Coombes, N., Bewley, K., Charlton, S., Walter, T. S., … Screaton, G. R. (2021). Evidence of escape of SARS-CoV-2 variant B.1.351 from natural and vaccine-induced sera. Cell, 184(9), 2348-2361.e6. https://doi.org/10.1016/j.cell.2021.02.037

2020 Publications

Ababneh, N. A., Scaber, J., Flynn, R., Douglas, A., Barbagallo, P., Candalija, A., Turner, M. R., Sims, D., Dafinca, R., Cowley, S. A., Cowley, S. A., & Talbot, K. (2020). Correction of amyotrophic lateral sclerosis related phenotypes in induced pluripotent stem cell-derived motor neurons carrying a hexanucleotide expansion mutation in C9orf72 by CRISPR/Cas9 genome editing using homology-directed repair. Human Molecular Genetics, 29(13), 2200–2217. https://doi.org/10.1093/hmg/ddaa106

Andersson, M., barnes, eleanor, Beneke, T., Crook, D., Downs, L., Eyre, D., Harvala, H., James, W., Justice, A., Klenerman, P., & others. (2020). SARS-CoV-2 RNA in blood.

Borrmann, H., Davies, R., Dickinson, M., Pedroza-Pacheco, I., Schilling, M., Vaughan-Jackson, A., Magri, A., James, W., Balfe, P., Borrow, P., McKeating, J. A., & Zhuang, X. (2020a). Pharmacological activation of the circadian component REV-ERB inhibits HIV-1 replication. Scientific Reports, 10(1), 13271. https://doi.org/10.1038/s41598-020-70170-3

Dafinca, R., Barbagallo, P., Farrimond, L., Candalija, A., Scaber, J., Ababneh, N. A., Sathyaprakash, C., Vowles, J., Cowley, S. A., & Talbot, K. (2020). Impairment of Mitochondrial Calcium Buffering Links Mutations in C9ORF72 and TARDBP in iPS-Derived Motor Neurons from Patients with ALS/FTD. Stem Cell Reports, 14(5), 892–908. https://doi.org/10.1016/j.stemcr.2020.03.023

Dodsworth, B. T., Hatje, K., Meyer, C. A., Flynn, R., & Cowley, S. A. (2020). Rates of homology directed repair of CRISPR-Cas9 induced double strand breaks are lower in naïve compared to primed human pluripotent stem cells. Stem Cell Research, 46. https://doi.org/10.1016/j.scr.2020.101852

Dodsworth, B. T., Hatje, K., Rostovskaya, M., Flynn, R., Meyer, C. A., & Cowley, S. A. (2020). Profiling of naïve and primed human pluripotent stem cells reveals state-associated miRNAs. Scientific Reports, 10(1). https://doi.org/10.1038/s41598-020-67376-w

Ebrahimi, K. H., Howie, D., Rowbotham, J., McCullagh, J., Armstrong, F., & James, W. S. (2020a). Viperin, through its radical‐SAM activity, depletes cellular nucleotide pools and interferes with mitochondrial metabolism to inhibit viral replication. FEBS Letters, 1873-3468.13761. https://doi.org/10.1002/1873-3468.13761

Folegatti, P. M., Ewer, K. J., Aley, P., Angus, B., Becker, S., Belij-Rammerstorfer, S., Bellamy, D., Bibi, S., Bittaye, M., Clutterbuck, E. . E. A., Dold, C., Faust, S. N., Finn, A., Flaxman, A. L., Hallis, B., Heath, P., Jenkin, D., Lazarus, R., Makinson, R., … Yau, Y. (2020). Safety and immunogenicity of the ChAdOx1 nCoV-19 vaccine against SARS-CoV-2: a preliminary report of a phase 1/2, single-blind, randomised controlled trial. The Lancet, 396(10249), 467–478. https://doi.org/https://doi.org/10.1016/S0140-6736(20)31604-4

Gutbier, S., Wanke, F., Dahm, N., Rümmelin, A., Zimmermann, S., Christensen, K., Köchl, F., Rautanen, A., Hatje, K., Geering, B., Cowley, S. A., & Patsch, C. (2020). Large-scale production of human IPSC-derived macrophages for drug screening. International Journal of Molecular Sciences, 21(13), 1–23. https://doi.org/10.3390/ijms21134808

Hall-Roberts, H., Agarwal, D., Obst, J., Smith, T. B., Monzón-Sandoval, J., di Daniel, E., Webber, C., James, W. S., Mead, E., Davis, J. B., & Cowley, S. A. (2020). TREM2 Alzheimer’s variant R47H causes similar transcriptional dysregulation to knockout, yet only subtle functional phenotypes in human iPSC-derived macrophages. Alzheimer’s Research and Therapy, 12(1), 1–27. https://doi.org/10.1186/s13195-020-00709-z

Hedegaard, A., Stodolak, S., James, W. S., & Cowley, S. A. (2020a). Honing the Double-Edged Sword: Improving Human iPSC-Microglia Models. Frontiers in Immunology, 11. https://doi.org/10.3389/fimmu.2020.614972

Helene, B., Rhianna, D., Dickinson, M., Isabela, P.-P., Mirjam, S., Vaughan-Jackson, A., Magri, A., James, W., Balfe, P., Persephone, B., others, Borrmann, H., Davies, R., Dickinson, M., Pedroza-Pacheco, I., Schilling, M., Vaughan-Jackson, A., Magri, A., James, W., … Zhuang, X. (2020). Pharmacological activation of the circadian component REV-ERB inhibits HIV-1 replication. Scientific Reports (Nature Publisher Group), 10(1), 13271. https://doi.org/10.1038/s41598-020-70170-3

Honarmand Ebrahimi, K., Rowbotham, J. S., McCullagh, J., & James, W. S. (2020a). Mechanism of Diol Dehydration by a Promiscuous Radical-SAM Enzyme Homologue of the Antiviral Enzyme Viperin (RSAD2). ChemBioChem, 21(11), 1605–1612. https://doi.org/10.1002/cbic.201900776

Honarmand Ebrahimi, K., Vowles, J., Browne, C., McCullagh, J., & James, W. S. (2020). ddhCTP produced by the radical-SAM activity of RSAD2 (viperin) inhibits the NAD+-dependent activity of enzymes to modulate metabolism. FEBS Letters, 594(10), 1631–1644. https://doi.org/10.1002/1873-3468.13778

Huo, J., le Bas, A., Ruza, R., Duyvesteyn, H. M. E., Mikolajek, H., Malinauskas, T., Tan, T. K., Rijal, P., Dumoux, M., Ward, P. N., Ren, J., Zhou, D., Harrison, P. J., Weckener, M., Clare, D. K., Vogirala, V. K., Radecke, J., Moynié, L., Zhao, Y., … Naismith, J. H. J. H. J. H. (2020a). Neutralizing nanobodies bind SARS-CoV-2 spike RBD and block interaction with ACE2. Nature Structural and Molecular Biology, 27(9), 1–9. https://doi.org/10.1038/s41594-020-0469-6

Lee, H., Flynn, R., Sharma, I., Haberman, E., Carling, P. J., Nicholls, F. J., Stegmann, M., Vowles, J., Haenseler, W., Wade-Martins, R., James, W. S., & Cowley, S. A. (2020a). LRRK2 Is Recruited to Phagosomes and Co-recruits RAB8 and RAB10 in Human Pluripotent Stem Cell-Derived Macrophages. Stem Cell Reports, 14(5), 940–955. https://doi.org/10.1016/j.stemcr.2020.04.001

Matthews, P. C., Andersson, M. I., Arancibia-Carcamo, C. V., Auckland, K., Baillie, J. K., Barnes, E., Beneke, T., Bibi, S., Brooks, T., Carroll, M., Wareing, S., Zambon, M., Crook, D., Dingle, K., Dold, C., Downs, L. O., Dunn, L., Eyre, D. W., Gilbert Jaramillo, J., … Zambon, M. (2020). SARS-CoV-2 RNA detected in blood products from patients with COVID-19 is not associated with infectious virus [version 1; peer review: 1 approved with reservations]. Wellcome Open Research, 5(181), 181. https://doi.org/10.12688/wellcomeopenres.16002.1

Miao, X., Luo, Y., Huang, X., Lee, S. M. Y., Yuan, Z., Tang, Y., Chen, L., Wang, C., Wu, F., Xu, Y. Y., Jiang, W., Gao, W., Song, X., Yan, Y., Pang, T., Chen, C.-P., Zou, Y., Fu, W., Wan, L., … Xu, Y. Y. (2020). A novel biparatopic hybrid antibody-ACE2 fusion that blocks SARS-CoV-2 infection: implications for therapy. MAbs, 12(1), 1804241. https://doi.org/10.1080/19420862.2020.1804241

Muliyil, S., Levet, C., Düsterhöft, S., Dulloo, I., Cowley, S. A., & Freeman, M. (2020). ADAM17-triggered TNF signalling protects the ageing Drosophila retina from lipid droplet-mediated degeneration. EMBO Journal, 39(17). https://doi.org/10.15252/embj.2020104415

Roberts, H. H., Agarwal, D., Daniel, E. di, Webber, C., James, W. S., Mead, E., Davis, J. B., & Cowley, S. A. (2020). TREM2 Alzheimer’s variant R47H causes similar transcriptional dysregulation to TREM2 knockout in human IPSC-derived macrophages: Developing topics. Alzheimer’s \& Dementia, 16, e047388.

Sanghani, H. R., Jagannath, A., Humberstone, T., Ebrahimjee, F., Thomas, J. M., Churchill, G. C., Cipriani, A., Attenburrow, M.-J., Perestenko, O. V., Cowley, S. A., Goodwin, G. M., & Vasudevan, S. R. (2020). Patient fibroblast circadian rhythms predict lithium sensitivity in bipolar disorder. Molecular Psychiatry. https://doi.org/10.1038/s41380-020-0769-6

Toombs, J., Panther, L., Ornelas, L., Liu, C., Gomez, E., Martín-Ibáñez, R., Cox, S. R., Ritchie, S. J., Harris, S. E., Taylor, A., Spires-Jones, T. L., & Sareen, D. (2020). Generation of twenty four induced pluripotent stem cell lines from twenty four members of the Lothian Birth Cohort 1936. Stem Cell Research, 46. https://doi.org/10.1016/j.scr.2020.101851

Wing, P. A., Keeley, T. P., Zhuang, X., Lee, J., Prange-Barczynska, M., Tsukuda, S., Morgan, S., Argles, I. la, Kurlekar, S., Noerenberg, M., Thompson, C. P., Huang, K.-Y. A., Balfe, P., Watashi, K., Castello Palomares, A., Hinks, T. S., James, W., Ratcliffe, P., Davies, I., … Mckeating, J. A. (2020). Hypoxic and Pharmacological Activation of HIF Inhibits SARS-CoV-2 Infection of Lung Epithelial Cells. SSRN Electronic Journal. https://doi.org/10.2139/ssrn.3720002

Zhou, D., Duyvesteyn, H. M. E., Chen, C.-P., Huang, C.-G., Chen, T.-H., Shih, S.-R. ., Lin, Y.-C., Cheng, C.-Y., Cheng, S.-H., Huang, Y.-C., Stuart, D. I., Huang, K.-Y. A., Lin, T.-Y., Ma, C., Huo, J., Carrique, L., Malinauskas, T., Ruza, R. R., Shah, P. N. M., … Huang, K.-Y. A. (2020). Structural basis for the neutralization of SARS-CoV-2 by an antibody from a convalescent patient. Nature Structural & Molecular Biology, 27(10), 950–958. https://doi.org/10.1038/s41594-020-0480-y