Recent Publications

All Since 2010

1: Huo J, Le Bas A, Ruza RR, Duyvesteyn HME, Mikolajek H, Malinauskas T, Tan TK,
Rijal P, Dumoux M, Ward PN, Ren J, Zhou D, Harrison PJ, Weckener M, Clare DK,
Vogirala VK, Radecke J, Moynié L, Zhao Y, Gilbert-Jaramillo J, Knight ML, Tree
JA, Buttigieg KR, Coombes N, Elmore MJ, Carroll MW, Carrique L, Shah PNM, James
W, Townsend AR, Stuart DI, Owens RJ, Naismith JH. Author Correction:
Neutralizing nanobodies bind SARS-CoV-2 spike RBD and block interaction with
ACE2. Nat Struct Mol Biol. 2020 Oct 15:1. doi: 10.1038/s41594-020-00527-9. Epub
ahead of print. Erratum for: Nat Struct Mol Biol. 2020 Sep;27(9):846-854. PMID:
33060850; PMCID: PMC7557317.

2: Miao X, Luo Y, Huang X, Lee SMY, Yuan Z, Tang Y, Chen L, Wang C, Wu F, Xu Y,
Jiang W, Gao W, Song X, Yan Y, Pang T, Chen C, Zou Y, Fu W, Wan L, Gilbert-
Jaramillo J, Knight M, Tan TK, Rijal P, Townsend A, Sun J, Liu X, James W, Tsun
A, Xu Y. A novel biparatopic hybrid antibody-ACE2 fusion that blocks SARS-CoV-2
infection: implications for therapy. MAbs. 2020 Jan-Dec;12(1):1804241. doi:
10.1080/19420862.2020.1804241. PMID: 32804015; PMCID: PMC7531490.

3: Borrmann H, Davies R, Dickinson M, Pedroza-Pacheco I, Schilling M, Vaughan-
Jackson A, Magri A, James W, Balfe P, Borrow P, McKeating JA, Zhuang X.
Pharmacological activation of the circadian component REV-ERB inhibits HIV-1
replication. Sci Rep. 2020 Aug 6;10(1):13271. doi: 10.1038/s41598-020-70170-3.
PMID: 32764708; PMCID: PMC7413328.

4: Zhou D, Duyvesteyn HME, Chen CP, Huang CG, Chen TH, Shih SR, Lin YC, Cheng
CY, Cheng SH, Huang YC, Lin TY, Ma C, Huo J, Carrique L, Malinauskas T, Ruza RR,
Shah PNM, Tan TK, Rijal P, Donat RF, Godwin K, Buttigieg KR, Tree JA, Radecke J,
Paterson NG, Supasa P, Mongkolsapaya J, Screaton GR, Carroll MW, Gilbert-
Jaramillo J, Knight ML, James W, Owens RJ, Naismith JH, Townsend AR, Fry EE,
Zhao Y, Ren J, Stuart DI, Huang KA. Structural basis for the neutralization of
SARS-CoV-2 by an antibody from a convalescent patient. Nat Struct Mol Biol. 2020
Oct;27(10):950-958. doi: 10.1038/s41594-020-0480-y. Epub 2020 Jul 31. PMID:
32737466.

5: Muliyil S, Levet C, Düsterhöft S, Dulloo I, Cowley SA, Freeman M.
ADAM17-triggered TNF signalling protects the ageing Drosophila retina from lipid
droplet-mediated degeneration. EMBO J. 2020 Sep 1;39(17):e104415. doi:
10.15252/embj.2020104415. Epub 2020 Jul 26. PMID: 32715522; PMCID: PMC7459420.

6: Huo J, Le Bas A, Ruza RR, Duyvesteyn HME, Mikolajek H, Malinauskas T, Tan TK,
Rijal P, Dumoux M, Ward PN, Ren J, Zhou D, Harrison PJ, Weckener M, Clare DK,
Vogirala VK, Radecke J, Moynié L, Zhao Y, Gilbert-Jaramillo J, Knight ML, Tree
JA, Buttigieg KR, Coombes N, Elmore MJ, Carroll MW, Carrique L, Shah PNM, James
W, Townsend AR, Stuart DI, Owens RJ, Naismith JH. Neutralizing nanobodies bind
SARS-CoV-2 spike RBD and block interaction with ACE2. Nat Struct Mol Biol. 2020
Sep;27(9):846-854. doi: 10.1038/s41594-020-0469-6. Epub 2020 Jul 13. Erratum in:
Nat Struct Mol Biol. 2020 Oct 15;: PMID: 32661423.

7: Gutbier S, Wanke F, Dahm N, Rümmelin A, Zimmermann S, Christensen K, Köchl F,
Rautanen A, Hatje K, Geering B, Zhang JD, Britschgi M, Cowley SA, Patsch C.
Large-Scale Production of Human iPSC-Derived Macrophages for Drug Screening. Int
J Mol Sci. 2020 Jul 7;21(13):4808. doi: 10.3390/ijms21134808. PMID: 32645954;
PMCID: PMC7370446.

8: Dodsworth BT, Hatje K, Rostovskaya M, Flynn R, Meyer CA, Cowley SA. Profiling
of naïve and primed human pluripotent stem cells reveals state-associated
miRNAs. Sci Rep. 2020 Jun 29;10(1):10542. doi: 10.1038/s41598-020-67376-w. PMID:
32601281; PMCID: PMC7324611.

9: Dodsworth BT, Hatje K, Meyer CA, Flynn R, Cowley SA. Rates of homology
directed repair of CRISPR-Cas9 induced double strand breaks are lower in naïve
compared to primed human pluripotent stem cells. Stem Cell Res. 2020
Jul;46:101852. doi: 10.1016/j.scr.2020.101852. Epub 2020 Jun 1. PMID: 32521498;
PMCID: PMC7347009.

10: Ababneh NA, Scaber J, Flynn R, Douglas A, Barbagallo P, Candalija A, Turner
MR, Sims D, Dafinca R, Cowley SA, Talbot K. Correction of amyotrophic lateral
sclerosis related phenotypes in induced pluripotent stem cell-derived motor
neurons carrying a hexanucleotide expansion mutation in C9orf72 by CRISPR/Cas9
genome editing using homology-directed repair. Hum Mol Genet. 2020 Aug
3;29(13):2200-2217. doi: 10.1093/hmg/ddaa106. PMID: 32504093; PMCID: PMC7399532.

11: Toombs J, Panther L, Ornelas L, Liu C, Gomez E, Martín-Ibáñez R, Cox SR,
Ritchie SJ, Harris SE, Taylor A, Redmond P, Russ TC, Murphy L, Cooper JD, Burr
K, Selvaraj BT, Browne C, Svendsen CN, Cowley SA, Deary IJ, Chandran S, Spires-
Jones TL, Sareen D. Generation of twenty four induced pluripotent stem cell
lines from twenty four members of the Lothian Birth Cohort 1936. Stem Cell Res.
2020 Jul;46:101851. doi: 10.1016/j.scr.2020.101851. Epub 2020 May 20. PMID:
32450543; PMCID: PMC7347008.

12: Sanghani HR, Jagannath A, Humberstone T, Ebrahimjee F, Thomas JM, Churchill
GC, Cipriani A, Attenburrow MJ, Perestenko OV, Cowley SA, Cader MZ, Peirson SN,
Harrison PJ, Foster RG, Goodwin GM, Vasudevan SR. Patient fibroblast circadian
rhythms predict lithium sensitivity in bipolar disorder. Mol Psychiatry. 2020
May 13. doi: 10.1038/s41380-020-0769-6. Epub ahead of print. PMID: 32404948.

13: Lee H, Flynn R, Sharma I, Haberman E, Carling PJ, Nicholls FJ, Stegmann M,
Vowles J, Haenseler W, Wade-Martins R, James WS, Cowley SA. LRRK2 Is Recruited
to Phagosomes and Co-recruits RAB8 and RAB10 in Human Pluripotent Stem Cell-
Derived Macrophages. Stem Cell Reports. 2020 May 12;14(5):940-955. doi:
10.1016/j.stemcr.2020.04.001. Epub 2020 Apr 30. PMID: 32359446; PMCID:
PMC7221108.

14: Dafinca R, Barbagallo P, Farrimond L, Candalija A, Scaber J, Ababneh NA,
Sathyaprakash C, Vowles J, Cowley SA, Talbot K. Impairment of Mitochondrial
Calcium Buffering Links Mutations in C9ORF72 and TARDBP in iPS-Derived Motor
Neurons from Patients with ALS/FTD. Stem Cell Reports. 2020 May
12;14(5):892-908. doi: 10.1016/j.stemcr.2020.03.023. Epub 2020 Apr 23. PMID:
32330447; PMCID: PMC7220989.

15: Honarmand Ebrahimi K, Vowles J, Browne C, McCullagh J, James WS. ddhCTP
produced by the radical-SAM activity of RSAD2 (viperin) inhibits the
NAD+ -dependent activity of enzymes to modulate metabolism. FEBS
Lett. 2020 May;594(10):1631-1644. doi: 10.1002/1873-3468.13778. Epub 2020 Apr13PMID: 32232843.

16: Ebrahimi KH, Howie D, Rowbotham JS, McCullagh J, Armstrong FA, James WS.
Viperin, through its radical-SAM activity, depletes cellular nucleotide pools
and interferes with mitochondrial metabolism to inhibit viral replication. FEBS
Lett. 2020 May;594(10):1624-1630. doi: 10.1002/1873-3468.13761. Epub 2020 Mar 2.
PMID: 32061099.

17: Honarmand Ebrahimi K, Rowbotham JS, McCullagh J, James WS. Mechanism of Diol
Dehydration by a Promiscuous Radical-SAM Enzyme Homologue of the Antiviral
Enzyme Viperin (RSAD2). Chembiochem. 2020 Jun 2;21(11):1605-1612. doi:
10.1002/cbic.201900776. Epub 2020 Feb 27. PMID: 31951306.

18: Pettingill P, Weir GA, Wei T, Wu Y, Flower G, Lalic T, Handel A, Duggal G,
Chintawar S, Cheung J, Arunasalam K, Couper E, Haupt LM, Griffiths LR, Bassett
A, Cowley SA, Cader MZ. A causal role for TRESK loss of function in migraine
mechanisms. Brain. 2019 Dec 1;142(12):3852-3867. doi: 10.1093/brain/awz342.
PMID: 31742594; PMCID: PMC6906598.

19: Überbacher C, Obergasteiger J, Volta M, Venezia S, Müller S, Pesce I, Pizzi
S, Lamonaca G, Picard A, Cattelan G, Malpeli G, Zoli M, Beccano-Kelly D, Flynn
R, Wade-Martins R, Pramstaller PP, Hicks AA, Cowley SA, Corti C. Application of
CRISPR/Cas9 editing and digital droplet PCR in human iPSCs to generate novel
knock-in reporter lines to visualize dopaminergic neurons. Stem Cell Res. 2019
Dec;41:101656. doi: 10.1016/j.scr.2019.101656. Epub 2019 Nov 9. PMID: 31733438;
PMCID: PMC7322529.

20: Booth HDE, Wessely F, Connor-Robson N, Rinaldi F, Vowles J, Browne C, Evetts
SG, Hu MT, Cowley SA, Webber C, Wade-Martins R. RNA sequencing reveals MMP2 and
TGFB1 downregulation in LRRK2 G2019S Parkinson’s iPSC-derived astrocytes.
Neurobiol Dis. 2019 Sep;129:56-66. doi: 10.1016/j.nbd.2019.05.006. Epub 2019 May11PMID: 31085228.

21: Connor-Robson N, Booth H, Martin JG, Gao B, Li K, Doig N, Vowles J, Browne
C, Klinger L, Juhasz P, Klein C, Cowley SA, Bolam P, Hirst W, Wade-Martins R. An
integrated transcriptomics and proteomics analysis reveals functional endocytic
dysregulation caused by mutations in LRRK2. Neurobiol Dis. 2019 Jul;127:512-526.
doi: 10.1016/j.nbd.2019.04.005. Epub 2019 Apr 5. PMID: 30954703; PMCID:
PMC6597903.

22: Jarosz-Griffiths HH, Corbett NJ, Rowland HA, Fisher K, Jones AC, Baron J,
Howell GJ, Cowley SA, Chintawar S, Cader MZ, Kellett KAB, Hooper NM. Proteolytic
shedding of the prion protein via activation of metallopeptidase ADAM10 reduces
cellular binding and toxicity of amyloid-β oligomers. J Biol Chem. 2019 Apr
26;294(17):7085-7097. doi: 10.1074/jbc.RA118.005364. Epub 2019 Mar 14. PMID:
30872401; PMCID: PMC6497954.

23: Gilbert-Jaramillo J, Garcez P, James W, Molnár Z, Clarke K. The potential
contribution of impaired brain glucose metabolism to congenital Zika syndrome. J
Anat. 2019 Sep;235(3):468-480. doi: 10.1111/joa.12959. Epub 2019 Feb 21. PMID:
30793304; PMCID: PMC6704275.

24: Zambon F, Cherubini M, Fernandes HJR, Lang C, Ryan BJ, Volpato V, Bengoa-
Vergniory N, Vingill S, Attar M, Booth HDE, Haenseler W, Vowles J, Bowden R,
Webber C, Cowley SA, Wade-Martins R. Cellular α-synuclein pathology is
associated with bioenergetic dysfunction in Parkinson’s iPSC-derived dopamine
neurons. Hum Mol Genet. 2019 Jun 15;28(12):2001-2013. doi: 10.1093/hmg/ddz038.
PMID: 30753527; PMCID: PMC6548224.

25: Nizami S, Hall-Roberts H, Warrier S, Cowley SA, Di Daniel E. Microglial
inflammation and phagocytosis in Alzheimer’s disease: Potential therapeutic
targets. Br J Pharmacol. 2019 Sep;176(18):3515-3532. doi: 10.1111/bph.14618.
Epub 2019 May 11. PMID: 30740661; PMCID: PMC6715590.

26: Lang C, Campbell KR, Ryan BJ, Carling P, Attar M, Vowles J, Perestenko OV,
Bowden R, Baig F, Kasten M, Hu MT, Cowley SA, Webber C, Wade-Martins R. Single-
Cell Sequencing of iPSC-Dopamine Neurons Reconstructs Disease Progression and
Identifies HDAC4 as a Regulator of Parkinson Cell Phenotypes. Cell Stem Cell.
2019 Jan 3;24(1):93-106.e6. doi: 10.1016/j.stem.2018.10.023. Epub 2018 Nov 29.
PMID: 30503143; PMCID: PMC6327112.

27: Buchrieser J, Oliva-Martin MJ, Moore MD, Long JCD, Cowley SA, Perez-Simón
JA, James W, Venero JL. RIPK1 is a critical modulator of both tonic and TLR-
responsive inflammatory and cell death pathways in human macrophage
differentiation. Cell Death Dis. 2018 Sep 24;9(10):973. doi:
10.1038/s41419-018-1053-4. PMID: 30250197; PMCID: PMC6155173.

28: Wong MMK, Hoekstra SD, Vowles J, Watson LM, Fuller G, Németh AH, Cowley SA,
Ansorge O, Talbot K, Becker EBE. Neurodegeneration in SCA14 is associated with
increased PKCγ kinase activity, mislocalization and aggregation. Acta
Neuropathol Commun. 2018 Sep 24;6(1):99. doi: 10.1186/s40478-018-0600-7. PMID:
30249303; PMCID: PMC6151931.

29: Rakovic A, Domingo A, Grütz K, Kulikovskaja L, Capetian P, Cowley SA, Lenz
I, Brüggemann N, Rosales R, Jamora D, Rolfs A, Seibler P, Westenberger A, König
I, Klein C. Genome editing in induced pluripotent stem cells rescues TAF1 levels
in X-linked dystonia-parkinsonism. Mov Disord. 2018 Jul;33(7):1108-1118. doi:
10.1002/mds.27441. PMID: 30153385.

30: Künzel U, Grieve AG, Meng Y, Sieber B, Cowley SA, Freeman M. FRMD8 promotes
inflammatory and growth factor signalling by stabilising the iRhom/ADAM17
sheddase complex. Elife. 2018 Jun 13;7:e35012. doi: 10.7554/eLife.35012. PMID:
29897336; PMCID: PMC6042961.

31: Härtlova A, Herbst S, Peltier J, Rodgers A, Bilkei-Gorzo O, Fearns A, Dill
BD, Lee H, Flynn R, Cowley SA, Davies P, Lewis PA, Ganley IG, Martinez J, Alessi
DR, Reith AD, Trost M, Gutierrez MG. LRRK2 is a negative regulator of
Mycobacterium tuberculosis phagosome maturation in macrophages. EMBO J.
2018 Jun 15;37(12):e98694. doi: 10.15252/embj.201798694. Epub 2018 May 22. PMID:
29789389; PMCID: PMC6003659.

32: Jonikas M, Madill M, Mathy A, Zekoll T, Zois CE, Wigfield S, Kurzawa-Akanbi
M, Browne C, Sims D, Chinnery PF, Cowley SA, Tofaris GK. Stem cell modeling of
mitochondrial parkinsonism reveals key functions of OPA1. Ann Neurol. 2018
May;83(5):915-925. doi: 10.1002/ana.25221. Epub 2018 Apr 25. PMID: 29604226;
PMCID: PMC6032926.

33: Watson LM, Wong MMK, Vowles J, Cowley SA, Becker EBE. A Simplified Method
for Generating Purkinje Cells from Human-Induced Pluripotent Stem Cells.
Cerebellum. 2018 Aug;17(4):419-427. doi: 10.1007/s12311-017-0913-2. PMID:
29397531; PMCID: PMC6028833.

34: Haenseler W, Kuzmenko E, Smalls-Mantey A, Browne C, Seger R, James WS,
Cowley SA, Reichenbach J, Siler U. Lentiviral gene therapy vector with UCOE
stably restores function in iPSC-derived neutrophils of a CDG patient. Matters
(Zur). 2018;2018:10.19185/matters.201805000005. doi:
10.19185/matters.201805000005. Epub 2018 May 23. PMID: 31008103; PMCID:
PMC6472893.

35: Heman-Ackah SM, Manzano R, Hoozemans JJM, Scheper W, Flynn R, Haerty W,
Cowley SA, Bassett AR, Wood MJA. Alpha-synuclein induces the unfolded protein
response in Parkinson’s disease SNCA triplication iPSC-derived neurons. Hum Mol
Genet. 2017 Nov 15;26(22):4441-4450. doi: 10.1093/hmg/ddx331. PMID: 28973645;
PMCID: PMC5886237.

36: Haenseler W, Zambon F, Lee H, Vowles J, Rinaldi F, Duggal G, Houlden H,
Gwinn K, Wray S, Luk KC, Wade-Martins R, James WS, Cowley SA. Excess α-synuclein
compromises phagocytosis in iPSC-derived macrophages. Sci Rep. 2017 Aug
21;7(1):9003. doi: 10.1038/s41598-017-09362-3. PMID: 28827786; PMCID:
PMC5567139.

37: Beevers JE, Lai MC, Collins E, Booth HDE, Zambon F, Parkkinen L, Vowles J,
Cowley SA, Wade-Martins R, Caffrey TM. MAPT Genetic Variation and Neuronal
Maturity Alter Isoform Expression Affecting Axonal Transport in iPSC-Derived
Dopamine Neurons. Stem Cell Reports. 2017 Aug 8;9(2):587-599. doi:
10.1016/j.stemcr.2017.06.005. Epub 2017 Jul 6. PMID: 28689993; PMCID:
PMC5549835.

38: Haenseler W, Sansom SN, Buchrieser J, Newey SE, Moore CS, Nicholls FJ,
Chintawar S, Schnell C, Antel JP, Allen ND, Cader MZ, Wade-Martins R, James WS,
Cowley SA. A Highly Efficient Human Pluripotent Stem Cell Microglia Model
Displays a Neuronal-Co-culture-Specific Expression Profile and Inflammatory
Response. Stem Cell Reports. 2017 Jun 6;8(6):1727-1742. doi:
10.1016/j.stemcr.2017.05.017. PMID: 28591653; PMCID: PMC5470330.

39: Lee H, James WS, Cowley SA. LRRK2 in peripheral and central nervous system
innate immunity: its link to Parkinson’s disease. Biochem Soc Trans. 2017 Feb
8;45(1):131-139. doi: 10.1042/BST20160262. PMID: 28202666; PMCID: PMC5652224.

40: Baud A, Wessely F, Mazzacuva F, McCormick J, Camuzeaux S, Heywood WE, Little
D, Vowles J, Tuefferd M, Mosaku O, Lako M, Armstrong L, Webber C, Cader MZ,
Peeters P, Gissen P, Cowley SA, Mills K. Multiplex High-Throughput Targeted
Proteomic Assay To Identify Induced Pluripotent Stem Cells. Anal Chem. 2017 Feb
21;89(4):2440-2448. doi: 10.1021/acs.analchem.6b04368. Epub 2017 Feb 6. PMID:
28192931.

41: Buchrieser J, James W, Moore MD. Human Induced Pluripotent Stem Cell-Derived
Macrophages Share Ontogeny with MYB-Independent Tissue-Resident Macrophages.
Stem Cell Reports. 2017 Feb 14;8(2):334-345. doi: 10.1016/j.stemcr.2016.12.020.
Epub 2017 Jan 19. PMID: 28111278; PMCID: PMC5312255.

42: Sandor C, Robertson P, Lang C, Heger A, Booth H, Vowles J, Witty L, Bowden
R, Hu M, Cowley SA, Wade-Martins R, Webber C. Transcriptomic profiling of
purified patient-derived dopamine neurons identifies convergent perturbations
and therapeutics for Parkinson’s disease. Hum Mol Genet. 2017 Feb
1;26(3):552-566. doi: 10.1093/hmg/ddw412. PMID: 28096185; PMCID: PMC5409122.

43: Wills QF, Mellado-Gomez E, Nolan R, Warner D, Sharma E, Broxholme J, Wright
B, Lockstone H, James W, Lynch M, Gonzales M, West J, Leyrat A, Padilla-Parra S,
Filippi S, Holmes C, Moore MD, Bowden R. The nature and nurture of cell
heterogeneity: accounting for macrophage gene-environment interactions with
single-cell RNA-Seq. BMC Genomics. 2017 Jan 7;18(1):53. doi:
10.1186/s12864-016-3445-0. PMID: 28061811; PMCID: PMC5219790.

44: Vazquez-Arango P, Vowles J, Browne C, Hartfield E, Fernandes HJ, Mandefro B,
Sareen D, James W, Wade-Martins R, Cowley SA, Murphy S, O’Reilly D. Variant U1
snRNAs are implicated in human pluripotent stem cell maintenance and
neuromuscular disease. Nucleic Acids Res. 2016 Dec 15;44(22):10960-10973. doi:
10.1093/nar/gkw711. Epub 2016 Aug 17. PMID: 27536002; PMCID: PMC5159530.

45: Kapellos TS, Taylor L, Lee H, Cowley SA, James WS, Iqbal AJ, Greaves DR. A
novel real time imaging platform to quantify macrophage phagocytosis. Biochem
Pharmacol. 2016 Sep 15;116:107-19. doi: 10.1016/j.bcp.2016.07.011. Epub 2016 Jul PMID: 27475716; PMCID: PMC5012892.

46: Dafinca R, Scaber J, Ababneh N, Lalic T, Weir G, Christian H, Vowles J,
Douglas AG, Fletcher-Jones A, Browne C, Nakanishi M, Turner MR, Wade-Martins R,
Cowley SA, Talbot K. C9orf72 Hexanucleotide Expansions Are Associated with
Altered Endoplasmic Reticulum Calcium Homeostasis and Stress Granule Formation
in Induced Pluripotent Stem Cell-Derived Neurons from Patients with Amyotrophic
Lateral Sclerosis and Frontotemporal Dementia. Stem Cells. 2016
Aug;34(8):2063-78. doi: 10.1002/stem.2388. Epub 2016 May 4. PMID: 27097283;
PMCID: PMC4979662.

47: Fernandes HJ, Hartfield EM, Christian HC, Emmanoulidou E, Zheng Y, Booth H,
Bogetofte H, Lang C, Ryan BJ, Sardi SP, Badger J, Vowles J, Evetts S, Tofaris
GK, Vekrellis K, Talbot K, Hu MT, James W, Cowley SA, Wade-Martins R. ER Stress
and Autophagic Perturbations Lead to Elevated Extracellular α-Synuclein in
GBA-N370S Parkinson’s iPSC-Derived Dopamine Neurons. Stem Cell Reports. 2016 Mar
8;6(3):342-56. doi: 10.1016/j.stemcr.2016.01.013. Epub 2016 Feb 18. PMID:
26905200; PMCID: PMC4788783.

48: Dodsworth BT, Flynn R, Cowley SA. The Current State of Naïve Human
Pluripotency. Stem Cells. 2015 Nov;33(11):3181-6. doi: 10.1002/stem.2085. Epub
2015 Jul 14. PMID: 26119873; PMCID: PMC4833179.

49: Flynn R, Grundmann A, Renz P, Hänseler W, James WS, Cowley SA, Moore MD.
CRISPR-mediated genotypic and phenotypic correction of a chronic granulomatous
disease mutation in human iPS cells. Exp Hematol. 2015 Oct;43(10):838-848.e3.
doi: 10.1016/j.exphem.2015.06.002. Epub 2015 Jun 19. PMID: 26101162; PMCID:
PMC4596252.

50: Hartfield EM, Yamasaki-Mann M, Ribeiro Fernandes HJ, Vowles J, James WS,
Cowley SA, Wade-Martins R. Physiological characterisation of human iPS-derived
dopaminergic neurons. PLoS One. 2014 Feb 21;9(2):e87388. doi:
10.1371/journal.pone.0087388. PMID: 24586273; PMCID: PMC3931621.

51: van Wilgenburg B, Moore MD, James WS, Cowley SA. The productive entry
pathway of HIV-1 in macrophages is dependent on endocytosis through lipid rafts
containing CD4. PLoS One. 2014 Jan 22;9(1):e86071. doi:
10.1371/journal.pone.0086071. PMID: 24465876; PMCID: PMC3899108.

52: van Wilgenburg B, Browne C, Vowles J, Cowley SA. Efficient, long term
production of monocyte-derived macrophages from human pluripotent stem cells
under partly-defined and fully-defined conditions. PLoS One. 2013 Aug
12;8(8):e71098. doi: 10.1371/journal.pone.0071098. PMID: 23951090; PMCID:
PMC3741356.

53: O’Reilly D, Dienstbier M, Cowley SA, Vazquez P, Drozdz M, Taylor S, James
WS, Murphy S. Differentially expressed, variant U1 snRNAs regulate gene
expression in human cells. Genome Res. 2013 Feb;23(2):281-91. doi:
10.1101/gr.142968.112. Epub 2012 Oct 15. PMID: 23070852; PMCID: PMC3561869.

54: Hartfield EM, Fernandes HJ, Vowles J, Cowley SA, Wade-Martins R. Cellular
reprogramming: a new approach to modelling Parkinson’s disease. Biochem Soc
Trans. 2012 Oct;40(5):1152-7. doi: 10.1042/BST20120159. PMID: 22988881.

55: Bernstone L, van Wilgenburg B, James W. Several commercially available anti-
CCR5 monoclonal antibodies lack specificity and should be used with caution.
Hybridoma (Larchmt). 2012 Feb;31(1):7-19. doi: 10.1089/hyb.2010.0092. PMID:
22316480.

56: Lufino MM, Popplestone AR, Cowley SA, Edser PA, James WS, Wade-Martins R.
Episomal transgene expression in pluripotent stem cells. Methods Mol Biol.
2011;767:369-87. doi: 10.1007/978-1-61779-201-4_27. PMID: 21822889.

57: Raposo RA, Trudgian DC, Thomas B, van Wilgenburg B, Cowley SA, James W.
Protein kinase C and NF-κB-dependent CD4 downregulation in macrophages induced
by T cell-derived soluble factors: consequences for HIV-1 infection. J Immunol.
2011 Jul 15;187(2):748-59. doi: 10.4049/jimmunol.1003678. Epub 2011 Jun 10.
PMID: 21666058; PMCID: PMC3587723.

58: Mandegar MA, Moralli D, Khoja S, Cowley S, Chan DY, Yusuf M, Mukherjee S,
Blundell MP, Volpi EV, Thrasher AJ, James W, Monaco ZL. Functional human
artificial chromosomes are generated and stably maintained in human embryonic
stem cells. Hum Mol Genet. 2011 Aug 1;20(15):2905-13. doi: 10.1093/hmg/ddr144.
Epub 2011 May 18. PMID: 21593218; PMCID: PMC3131039.

59: Raposo RA, Thomas B, Ridlova G, James W. Proteomic-based identification of
CD4-interacting proteins in human primary macrophages. PLoS One. 2011 Apr
13;6(4):e18690. doi: 10.1371/journal.pone.0018690. PMID: 21533244; PMCID:
PMC3076427.

60: Moore MD, Cookson J, Coventry VK, Sproat B, Rabe L, Cranston RD, McGowan I,
James W. Protection of HIV neutralizing aptamers against rectal and vaginal
nucleases: implications for RNA-based therapeutics. J Biol Chem. 2011 Jan
28;286(4):2526-35. doi: 10.1074/jbc.M110.178426. Epub 2010 Nov 24. PMID:
21106536; PMCID: PMC3024747.

61: Carter GC, Bernstone L, Baskaran D, James W. HIV-1 infects macrophages by
exploiting an endocytic route dependent on dynamin, Rac1 and Pak1. Virology.
2011 Jan 20;409(2):234-50. doi: 10.1016/j.virol.2010.10.018. Epub 2010 Nov 6.
PMID: 21056892.

62: Brook FA, Cowley SA, Evans EP, Turner K, James WS, Mardon HJ. Derivation and
characterisation of the human embryonic stem cell line, OxF1. In Vitro Cell Dev
Biol Anim. 2010 Apr;46(3-4):173-7. doi: 10.1007/s11626-010-9283-5. Epub 2010 Mar PMID: 20333479.

63: Xu X, Cowley S, Flaim CJ, James W, Seymour L, Cui Z. The roles of apoptotic
pathways in the low recovery rate after cryopreservation of dissociated human
embryonic stem cells. Biotechnol Prog. 2010 May-Jun;26(3):827-37. doi:
10.1002/btpr.368. PMID: 20077485; PMCID: PMC3596802.

64: Xu X, Cowley S, Flaim CJ, James W, Seymour LW, Cui Z. Enhancement of cell
recovery for dissociated human embryonic stem cells after cryopreservation.
Biotechnol Prog. 2010 May-Jun;26(3):781-8. doi: 10.1002/btpr.358. PMID:
20014103; PMCID: PMC3593169.