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qPCR Facility

We maintain two Applied BioSystems QuantStudio 5 qPCR machines on behalf of the department, which are available for general use, in the instrument room, OMPI2 510.30.27.

One is 96 well format and the other 384 well format. Iformation on these machines can be found at https://www.thermofisher.com/order/catalog/product/A28575?SID=srch-srp-A28575#/A28575?SID=srch-srp-A28575 and https://www.thermofisher.com/order/catalog/product/A28574?SID=srch-srp-A28574#/A28574?SID=srch-srp-A28574

The person in the JamesLab currently overseeing them is Maria, so if you wish to use them or report an issue, please let her know at mailto:maria.karabova@path.ox.ac.uk.

As a user, you are responsible for all consumable items, and must leave the instruments in a state fit for others to use.

Genome editing in induced pluripotent stem cells rescues TAF1 levels in X-linked dystonia-parkinsonism

Author(s):Rakovic, A. | Domingo, A. | Grütz, K. | Kulikovskaja, L. | Capetian, P. | Cowley, S.A. | Lenz, I. | Brüggemann, N. | Rosales, R. | Jamora, D. | Rolfs, A. | Seibler, P. | Westenberger, A. | König, I. | Klein, C.
Publication year: 2018
Journal / Book title: Movement Disorders

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Source:: cowley, sally a.” 7102482900 “james, william s.” 35479049300 “moore, michael d.

Variant U1 snRNAs are implicated in human pluripotent stem cell maintenance and neuromuscular disease

Author(s):Vazquez-Arango, P. | Vowles, J. | Browne, C. | Hartfield, E. | Fernandes, H.J.R. | Mandefro, B. | Sareen, D. | James, W. | Wade-Martins, R. | Cowley, S.A. | Murphy, S. | O’Reilly, D.
Publication year: 2016
Journal / Book title: Nucleic Acids Research

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Source:: cowley, sally a.” 7102482900 “james, william s.” 35479049300 “moore, michael d.

Multiplex High-Throughput Targeted Proteomic Assay to Identify Induced Pluripotent Stem Cells

Author(s):Baud, A. | Wessely, F. | Mazzacuva, F. | McCormick, J. | Camuzeaux, S. | Heywood, W.E. | Little, D. | Vowles, J. | Tuefferd, M. | Mosaku, O. | Lako, M. | Armstrong, L. | Webber, C. | Cader, M.Z. | Peeters, P. | Gissen, P. | Cowley, S.A. | Mills, K.
Publication year: 2017
Journal / Book title: Analytical Chemistry

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Source:: cowley, sally a.” 7102482900 “james, william s.” 35479049300 “moore, michael d.

A Highly Efficient Human Pluripotent Stem Cell Microglia Model Displays a Neuronal-Co-culture-Specific Expression Profile and Inflammatory Response

Author(s):Haenseler, W. | Sansom, S.N. | Buchrieser, J. | Newey, S.E. | Moore, C.S. | Nicholls, F.J. | Chintawar, S. | Schnell, C. | Antel, J.P. | Allen, N.D. | Cader, M.Z. | Wade-Martins, R. | James, W.S. | Cowley, S.A.
Publication year: 2017
Journal / Book title: Stem Cell Reports

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Source:: cowley, sally a.” 7102482900 “james, william s.” 35479049300 “moore, michael d.

Lopinavir plus nucleoside reverse-transcriptase inhibitors, lopinavir plus raltegravir, or lopinavir monotherapy for second-line treatment of HIV (EARNEST): 144-week follow-up results from a randomised controlled trial

Author(s):Hakim, J.G. | Thompson, J. | Kityo, C. | Hoppe, A. | Kambugu, A. | van Oosterhout, J.J. | Lugemwa, A. | Siika, A. | Mwebaze, R. | Mweemba, A. | Abongomera, G. | Thomason, M.J. | Easterbrook, P. | Mugyenyi, P. | Walker, A.S. | Paton, N.I. | Agweng, E. | Awio, P. | Bakeinyaga, G. | Isabirye, C. | Kabuga, U. | Kasuswa, S. | Katuramu, M. | Kiweewa, F. | Kyomugisha, H. …read more

Source:: cowley, sally a.” 7102482900 “james, william s.” 35479049300 “moore, michael d.

Stem cell modeling of mitochondrial parkinsonism reveals key functions of OPA1

Author(s):Jonikas, M. | Madill, M. | Mathy, A. | Zekoll, T. | Zois, C.E. | Wigfield, S. | Kurzawa-Akanbi, M. | Browne, C. | Sims, D. | Chinnery, P.F. | Cowley, S.A. | Tofaris, G.K.
Publication year: 2018
Journal / Book title: Annals of Neurology

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Source:: cowley, sally a.” 7102482900 “james, william s.” 35479049300 “moore, michael d.